Corn Sourdough with wild yeasts and S.bayanus


Come try this tasty bread at our event this Monday, January 27th, 7 to 9:00. We still have 9 seats left! Eventbrite (Register at this link, or at MeetUp for cash donations). With Chris Cuzme and Mary Izett that create their brews at Fifth Hammer Brewing Company in Long Island City, where the event is taking place. And try some things that we made using enzymes, and yeasts starters.

We typically have an ongoing sourdough starter that we feed at least once a day. We never throw out any of it because we have so many uses for it. It’s fermented dough.

We typically feed the starter based on the formula described below. If you are taking it out of the fridge where it has been stored for a while, you have to feed it at least 3 or four times before it will be active enough to raise your dough. That means at least 24 hours.

First, put the recently used starter into a clean jar and add the water. 75F degree water is best. Not hotter. Mix it very well. Then add the flour and mix. Cover tighly or not.

Keeping it at 72F to 85F is best unless you want to grow it more slowly. It typically takes about 3 hours to become active after each feeding. It should at least double in size. The most common failure to rise issue is that the starter is not active enough. Same with brewing.



  • 40 grams sourdough starter from a recent previous batch
  • 70 grams strong bread flour
  • 70 grams water at 75F

After you have started the process of getting your starter active, mix the flour and water that you are going to use for the bread. We like to let it sit, covered, in a warm spot for at least three hours as well.

We highly recommend you do this step. If you want you can mix your flour and water then refrigerate and bring to room temperature the next day. Or even several days later. This is called autolyze, a part of starch hydrolization that is very similar to the process called gelatinization.

  • 700 grams bread flour
  • 450 grams water


  • 640 grams corn biga with S. bayanus yeast
  • 140 grams active starter
  • 36 grams shio-koji (if omitted, use coarse sea salt as specified below)

A biga is made by mixing flour and water together with a small amount of yeast. It is then refrigerated overnight or longer. We used corn bran and rice bran for this biga.

The yeast we used was S.bayanus. This yeast is typically made for wine and beer brewing. You could use another yeast if you like.

We mix the biga and the sourdougb starter together very well, turning it onto itself in a bowl for several minutes.

We then took the mix of water and flour from several hours earlier and mixed that into the biga and sourdough starter mix. We did this while adding the shio koji.

We aimed for 2% salt in this bread, based on bakers percentages. That means that we added up all the flour we used including the flour that was used in the sourdough starter and the biga.

The total flour amount was 1100 grams. That means we needed 22 grams of salt or 170 grams of aged, salty shio-koji. We added 148 grams more of shio koji to the mix after we rested it for 30 minutes.

We kneaded dough, several times while letting it rise again. Finally, we put one half in the fridge to test the yeast – and the other half in a large loaf pan. It was lft to rise for 120 minutes.


9 AM
11 AM

Bake at 450 for 30 to 45 minutes. Remove from oven and let cool for at least 2 hours.

We already have some great ideas for some other yeasts. All baked goods should have one form of filamentous fungus (Aspergillus, Rhizopus, etc.) or bacterial enzymes or both in them. We already have some great ideas for some other yeasts.



Shio Koji (salt koji)


Shio koji can be kept in an air lock sealed jar but we prefer the refrigerator unless we are aging it. When aging shio koji we typically add some alcohol. We will have some quick pickles made with shio koji at Monday’s event.

  • 300 grams of koji (rice, barley, corn, wheat, etc.)
  • 100 grams of coarse salt
  • 400 grams of water

As you can see the golden rule of shio-koji making is a 3:1:4 ratio. You should try to make the salt equal 12.5% of the total weight of your shio-koji. By weight, not by volume.

The salt percentage of any shio-koji should be between 12 and 15%, but never exceed 15% or go below 7%. There are yeasts and microbes that can still live in a 7% salt solution. Over 15% and protease and other enzymes are denatured. You could still use it as a seasoning though.

If you are using 300 grams of koji, you massage that with 100 grams of salt. Just like when making miso you should always massage your koji and salt.


Quick pickles made with shio koji

If you wait an hour you will see a dramatic change. The temperature may even rise. That means your koji has active enzymes.

You can make this in a blender – our preference – but remember that any time you expose koji to mechanical action it will produce heat. Don’t make a lot at once, and chill your Vitamix or blender first.

After grinding or massaging the salt and koji add the water. You add 400 grams of water, cover tightly and place in a dark place for this recipe. Shake every day for 10 to 14 days.

Store in refrigeraor or at room temperature under air lock. Don’t make too much at a time, as it will become infected with wild yeasts and bacteria if you keep opening and closing the container.



When using shio koji to replace salt you should use 2 to 3 tsp to replace a tsp of salt.

A tablespoon of shio koji per pound of fish or meat to marinate for 15 minutes is enough. Usually 10 to 15% of the weight of whatever you are using the shio koji on will suffice.

There is sugar in shio koji so careful when you cook it. Wipe the shio koji off if you like. It’s excellent in baked goods.


Walnut Amasake Bittersweet Chocolate Muffins


Baking with microbes

Almost everything we bake, brew cook or ferment contains one or more microbes. Bacteria, yeasts, fungus and other fermented products that already contain microbes (like miso, milk kefir, and vinegar) work exceptionally well in and with baked goods.

Even if you set aside the yeasts common in bread baking, we almost always use shio-koji instead of salt, milk kefir or amasake instead of milk, and often lacto-fermented fruits,vegetables and even grains in baking.

Muffins and tea breads are basically are usually the same batter baked in different size baking pans. Obviously a bigger pan means a longer baking time, maybe 45 minutes as opposed to 30 minutes at 350F for the 8 big muffins that this recipe makes.


Ingredients

Our rules of muffin making as well as tea breads are simple.

  • The batter should be just barely mixed
  • The batter should be on the wetter side
  • Never fill a pan more than two thirds full
  • Add 1 tsp baking soda with the dry ingredients
  • Mix ins like nuts go with dry ingredients
  • Fruits and/or flavored essences or sauces go with wet stuff
  • Don’t mix in wet fruits or ferments until the end if color maters
  • Let muffin batter rest and puff up before spooning into cups

Half baked at 15 minutes. Looking good.

The recipe for these muffins pretty much follow the standard muffin ratio that every baker has memorized. Butter by weight equals sugar by weight. That combined weight is the weight of the flour. It’s also the weight in whatever measurement system you are using in liquid. In most cases add-ins like nuts or berries should never exceed in volume the sugar or flour volume.

Because we add a fermented or microbe inclusive ingredient to our baked goods – typically of a lower, acidic pH – we always add baking soda with the powder. Sourdough leavened muffins follow a different procedure based on bakers ratios that we’ll explain in another post.



  • 8 ounces or 1 1/2 cups or 236 grams all purpose flour or other
  • 4 ounces or 1/2 cup coconut palm sugar or other
  • 2 tsp baking powder
  • 4.3 ounces or 1 cup or 124 grams roasted chopped walnuts
  • 3.1 ounces or 1/2 cup or 90 grams bittersweet chocolate chips/chunks
  • 1.2 ounces or 2 TB or 32 grams shio-koji (or 1 tsp salt)
  • 8 ounces or 3/4 cup or 230 grams rice amasake (or nut or dairy milk)
  • 1 TB vanilla (or chocolate extract or mirin or soy sauce)
  • 4.5 ounces or 2 extra large or 126 grams eggs (or two vegan eggs)
  • 5 ounces or 1/2 cup or 156 grams dark maple syrup
  • 4 ounces or 1/2 cup or 112 grams roasted walnut oil (or butter/oil)


Procedure

  • Preheat oven to 350F.
  • Have bottom shelf ready for one or two muffin tins.
  • Prepare the tins with grease or just paper linings.
  • Mix flour, coconut palm sugar, baking powder, roasted chopped walnuts, bittersweet chocolate chips/chunks together
  • Whisk your eggs, walnut oil, maple syrup, vanilla, amasake, and shio-koji together well. Add to just incorporate with the dry ingredients.
  • Add add ins if you have reserved them to maintain the color or spacing of the add ins in your batter. *Check your chocolate to ensure it is non-dairy if vegan baking
  • Spoon about 4 ounces into each of 8 prepared muffin cups.
  • If using a smaller muffin tea or mini-muffin pans remember the rule – never more than 2/3 full.
  • Bake at 350F for 30 minutes in a pre-heated oven.
  • Don’t open the oven door until 20 minutes have elapsed.
  • Internal temperature should be 205 F, or a clean toothpick.
  • Remove muffins from oven and let rest 30 minutes away from heat.
  • Place on wire rack for further cooling after removing from the pan.


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Making Koji, Malting

Making shio-koji
Shio-koji. Also called salt koji. See below for instructions below on making it.

Shio

Spent rye grains inoculated with Aspergillus luchuensis and Rhizopus oryzae and other microbes for a sour rye liquid for baking and for brewing. That’s advanced level. Keep reading.


A fermentable sugar is something that a yeast or bacteria uses as an energy source. Grains (cereals) such as barley or millet or rice have a lot of starch. To be useful they have to be broken down into smaller pieces called simple sugars.

Filamentous fungus are a specific type of microbe capable of doing this. Either alone or in different combinations koji (Aspergillus and another specific fungus called Rhizopus) does this by creating enzymes that power or catalyse this process. They can break down lots of things.

Specific types of cultures or microbes that either include koji or are entirely made up of koji are used. They break things down into smaller pieces by creating many different types of enzymes.

Koji can break down starches, fats, proteins and other things from organic sources. Usually this is done through a process called hydrolysis, which just means water is involved in the process.

Koji (A. oryzae) is better at – and does not produce harmful toxins like a close relative Aspergillus flavus – at breaking things down. The breakdown enzymes that brewers are mostly concerned with are amylases, glucoamylases, pectinases, proteases, and lipases.

Koji is genetically and specifically capable of making hydrolytic enzymes and enzymes that move sugars and other substances around during fermentation. Remember that without enzymes everything would need more energy that would ever be available.

You can also use koji in it’s extracted form as well as a whole substrate such as koji grown on rice to make pickles, sake, shoyu koji, amasake and more.

Aspergillus oryzae has been specifically selected out over many years so that the strain we use typically knows what to do. When a great batch of sake was made, brewers used the same strain that had made that batch to create a new one with the same desirable koji properties.

Malting is done by sprouting or germinating a grain. Many different kinds can be used including rice, barley, and corn. All grains contain a lot of starch. The serve as a seeds energy source.

If you expose dried grains to water and the right temperature they will sprout, creating enzymes to break down starches for the grain to grow. This is called malting. Very useful for a plant until there are leaves that can get energy through photosynthesis. But brewers get the. enzymes before they are spent on growth. They are used to break down their starches.

As long as some fermentable sugars are available we create a moromi or wort to make alcohol. Whether you are making beer, sake, vinegar or soy sauce, wild yeasts or specifically selected yeasts turn the sugars into alcohol, carbon dioxide and heat.

The heat comes from the microbes doing their job has to be carefully controlled during koji making, as well as during the process of making beer or sake or another product. There are different ways this can be done, as there are different types of koji better for specific outcomes such as sake or shoyu。

But whether it’s sake or beer – which actually usually uses malted grains that produce the same type of enzymes as koji – the same saying applies to all microbrewing: “Ichi koji, ni moto, san zukuri”. First koji, second the Moto, third the fermentation.


We are having our last #KojiFest2019 event and the first #Zymes2020 event at Fifth Hammer Brewing in Long Island City on December 16th, 2019. You must pre-register for the event.

In the meantime, we will be publishing this 16 part series about how to make koji and extract it’s enzymes, and how to use what the koji is made on – a substrate – directly in brewing or baking or miso or sauce making.


About Enzymes

Enzymes serve two roles. They break down things such as tiny bits of food that you eat into smaller things. Or they combine smaller things like the amino acids from proteins into bigger things. Enzymes make possible every vital function of living things. Sometimes enzymes already exist in living things like your gut.

But most times they are used process things into food or drinks. Without the enzymes in malted barley, for example, it’s unlikely that either bread nor bread would exist as they do today.

Enzymes from koji have been used in European and American food manufacturing for at least 100 years now. We will get into what they have sometimes been combined with (other microbes, yeasts, techniques).

They are very safe to use, but you must be careful when handling them. Anything that can tear through grains or the muscles of animals should not be handled carelessly (see below).


Joichi Takamine

“Curiously enough this tiny and important hustler has scarcely attracted attention in the Occident, and this fact made me determine to work for it’s introduction to industrial use in the United States”

This quote is from a paper printed in 1914 in The Journal of Industrial and Engineering Chemistry (Vol.6, No.10). The author is Jokichi Takamine. He’s talking about koji (A.oryzae).

One of the things he was trying to do was to address the concerns of brewers and maltster – the people that make sprouted grain malt for beer – about the cost of the ingredients to create diastatic enzymes. The price of barley could vary. considerably from season to season. He had earlier filed a patent in the US to do so, “in a process not hitherto practiced”. He succeeded.


Takadiastase

On February 23, 1894, a patent was filed in the US : “The object of this invention is to prepare and manufacture diastatic enzyme, or soluble ferment in a concentrated form which possesses the power of transforming starch into’ sugar for use in various industries, by a process not hitherto practiced, and in a very economical and practical manner. 

My invention is based upon the utilization of the property possessed by certain fungi during their growth on proper media of producing diastatic enzyme.

Typically, barley was malted. Malting creates diastatic enzymes. But Takamine thought of using something that was pretty much being thrown away, yet which could produce way more diastatic power and be less perishable. 

Despite some violent outbursts suspected to have come from the maltsters at the time afraid of losing both money and their businesses, the brewing and food manufacturing industries in in the US ultimately adopted the use of enzymes, sometimes malt from barley or other grains for beer, but overwhelmingly from fungal enzymes from Aspergillus in baking, food processing and medicine.

Today both brewing supply houses and enzyme companies sell specific enzymes from many fungus, molds, even bacteria but different types of Aspergillus (koji) are used extensively throughout the world.

Takamine’s substance became an amazingly useful drug used to help people digest food. Enzymes are a very big deal. You can make your own as described below, but be aware that a small microbrewing operation can very quickly become larger than the brewing or refrigeration or heating capacity of your space.


How to Make Takadiastase
  • 5 gallon container
  • 2 1/4 pounds (1024 grams) wheat bran
  • 3 gallons lukewarm water (9000 grams)
  • 1/2 cup (138 grams) coarse 100% NaCl salt
  • Aspergillus oryzae spores (1 gram pure spores)

Taka-Diastase is made with Aspergillus oryzae, the hardest working fungus in the world. It is made on wheat bran. We milled off the bran from winter wheat berries – they have tasty bran, but other types of wheat brans work well – but you can buy it in sizes from one to 50 pounds.

We suggest you start off with 2 and 1/4 pounds (1024 grams) of wheat bran. Remember that wheat bran is unlike wheat berries or even flour. It is typically very light when dry and flies everywhere.

This is especially important when you are about to harvest (or dekoji) your finished Taka-Diastase. Unlike, say, rice koji, you have to consider how to reduce the temperature without fans after a certain point.

As the temperature decreases and it dries out, fans will blow it everywhere. I recommend either using wood that can absorb a lot of water – when you reduce the heat of something the water will either evaporate in the air or soak into whatever it is on.

We often use lots of cloths when making bean or rice koji. Whatever you chose, be prepared to have lots of cloth changes or at least one other wooden to container to transfer the koji into as it dries.

Unless you are going to use it almost as soon as it is finished – we sometimes have a salt brine ready to throw prepared koji into while still warm – you have to dry it out. Otherwise, it will continue to grow.

And a moist pile of amino acids are a feast for all the wild microbes that hang out in the air.

Did any of this seem complicated or overwhelming? Most people don’t ever make their own koji. You can easily buy it. In 35 pound boxes rice koji can be quite reasonably priced.

But if you only want to make a pound or two of koji, it’s cheaper making it yourself. You can also buy the powdered enzymes. But here are basic guidelines to make any type of koji. (Part 2, next post.)


The Awesome Power of Koji

There is actually a large body of evidence on why bread and pastry bakers, farmers, and koji growers must be very careful about both inhaling aspergillus spores, as well as getting it on their skin. We will talk about that as well during the year long series, just remember to always wears gloves and masks when dealing with enzymes. 

It makes sense that something with the power to break down meat, fish, or very hard grains would be something to treat with caution. Sometimes things that are obvious to someone working in a professional, including the pharmaceutical industry and food manufacturing industries where Aspergillus and enzymes are used extensively, are not well known or made public.

Please be careful when handling enzymes created by anything, either from a sprouted grain or a fungus. Here are is a very small sample of some of the things that can happen when inhaling spores or enzymes from Aspergillus oryzae. As in the koji we are describing how to make.

  • Valdivieso, R & Subiza, Jose & Hinojosa, Mariel & Carlos, E & Subiza, E. (1994). Baker’s asthma caused by alpha amylase. Annals of allergy. 73. 337-42.  Abstract: Two bakers with bronchial asthma and two with rhinoconjunctivitis are described. Prick and RAST tests were positive with wheat flour in all of them, but the challenge test (nasal or bronchial) with wheat flour extract was positive only in one asthmatic baker. The prick test, RAST, and nasal or bronchial challenge done with alpha amylase extract (a glycolytic enzyme obtained from Aspergillus oryzae and used as a flour additive) were positive in all four patients. Our results support previous data indicating that alpha amylase used in bakeries is an important antigen that could cause respiratory allergy in bakers. It can function as sole causative allergen or in addition with other allergens used in the baking industry.
  • Sharma BB, Singh S, Singh V. Hypersensitivity pneumonitis: the dug-well lung. Allergy Asthma Proc 2013;34:e59–64.
  • Gerfaud-Valentin M, Reboux G, Traclet J, et al. Occupational hypersensitivity pneumonitis in a baker: a new cause. Chest 2014;145:856–8.

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